Figure 1
Expression and localization of FSHR in human umbilical vein endothelial cells (HUVEC).
(A) FSHR expression was analysed with primers spanned different exons of FSHR using cDNA from primary HUVECs (passage 0), umbilical cord (UC), umbilical cord vein (UC vein), umbilical cord artery (UC artery), HUV-ST (SV40Tag/telomerase-immortalized human umbilical vein endothelial cell line) cell line and human granulosa cells passage 1, as a positive control. A no-reverse transcriptase control (-RT) and no template control (H2O) were used as negative controls. Beta-actin (B-actin, ACTB) was used as housekeeping gene. A representative picture of cropped gel has been shown here, full-length blots/gels are presented in Supplementary information file. (B) RNAScope In situ hybridization of FSHR was performed in umbilical cord and granulosa cell tumour formalin fixed paraffin embedded sections. POLR2A (lower panel), a positive control probe for low expression was used as a sections’ quality control. (C) Immunocytochemical co-localization of FSHR and CD31 in HUVECs and HUV-ST cells. Human granulosa cells and HEK293 cells stably transfected with human FSHR cDNA (HEK-FSHR cells) were used as FSHR-positive control cells. FOXL2 was used as a granulosa cell marker where FLAG peptide was used as a reporter sequence to detect FSHR construct in HEK-FSHR cells. DAPI was used as counterstaining to detect cell nuclei (blue).
