Figure 13

Cytotoxicity of various DDS formulations in MCF7 cells by XTT Assay: Cytotoxicity of various synthesized delivery vehicles containing selected equimolar doxorubicin such as (a) free doxorubicin (0.5 to 4 μM) (b) GNP^dox+, (c) ^FA−DDS^dox+, (d) ^FA+DDS^dox+, and (e) GNP^dox+:^FA+DDS^dox+ were evaluated on MCF7 cells for 8.0 h, 24, 48, and 72 hours. Cells were treated with calculated concentrations of these DDS (given along with each figure) and cell viability was measured in comparison to untreated control cells. ^FA−DDS^dox+ lacks targeting based internalization did not show any significant effect on cell viability. ^FA+DDS^dox+ and GNP^dox+:^FA+DDS^dox+ showed the cytotoxicity after 48 hours but slower rate than equimolar doxorubicin containing GNP^dox+ and free doxorubicin. The GNP^dox+ and free doxorubicin showed higher cell toxicity due to rapid diffusion into the cells. Results were presented as mean ± SD values run in triplicate and repeated.