Figure 1
Glycinergic neurotransmission is reduced in radial neurons of superficial dorsal horn after PNL.
(a) Neurons were classified by their morphology. Micrographs show representative examples of lucifer yellow filled neurons immediately following electrophysiological recording (top row) and biocytin filled neurons imaged by confocal microscopy (bottom row). All cells are shown in the parasagittal plane and the scale bar = 10 μm. (b) Examples of glycine-mediated eIPSC traces from control (sham, grey) and nerve-injured (PNL, red) spinal cord neurons. (c) Normalized glycinergic eIPSC values as a percentage of the total eIPSC for the neuron types (***p < 0.001, two way ANOVA, Sidak post-hoc multiple comparisons test). (d) Representative traces of total eIPSCs and GABA eIPSCs, respectively, from radial neurons of sham (grey, black) and PNL (pink, red) animals. Normalized GABA eIPSC values as a percentage of the total eIPSC from radial neurons of control and nerve injured animals. ***p < 0.001 (unpaired t test). (e) Representative traces from radial cells of control (black) and nerve-injured (red) animals with amplitudes normalized to the first pulse and fitted decay exponential shown in grey. Comparison of glycinergic eIPSC decay time constant from control and nerve injured groups (n =10 for each group) ***p < 0.001 (unpaired t tests).
