Figure 7
Cyclothiazide decreases glycinergic eIPSC amplitude in radial cells from nerve-injured animals.
(a) Western blot detection of GlyRα2 from membrane proteins from the lumbar dorsal horn of control and nerve-injured animals. Actin is used as a loading control. Scatter dot plot shows densitometry of GlyRα2 western blot bands normalized to the loading control and then the sham control. **p < 0.01 (unpaired t test). Full length blots are presented in Supplementary Figure 1. (b) Time plot showing normalized glycinergic eIPSC amplitudes from radial cells of control (red) and nerve injured (grey) animals in response to cylclothiazide (CTZ). Darker filled circles show time points where drug is superfused. Mean taken between 2–4 minutes (baseline) and 8–10 minutes (CTZ), normalized to the first 5 minutes of recording. (c) Plots of eIPSC response to CTZ from control and nerve-injured tissue. ***p < 0.001 (two way ANOVA with Tukey multiple comparisons test). (d) Representative trace and dot plot showing decrease in decay time following application of CTZ in radial cells from nerve-injured animals.**p < 0.01 (paired t tests).
