Table 3 Cellular acetyl-CoA and PHB synthase activity levels after heterotrophic culture in the dark.

From: Two-stage (photoautotrophy and heterotrophy) cultivation enables efficient production of bioplastic poly-3-hydroxybutyrate in auto-sedimenting cyanobacterium

Nutrient condition

Acetyl-CoA level (μg/g DW)

PHB synthase activity (nmol/min/mg protein)

Act 0.1% (w/v)

0.2% (w/v)

0.1% (w/v)

0.2% (w/v)

NORMAL

25.2 ± 5.1

20.3 ± 6.1

15.2 ± 4.2

13.2 ± 4.1

-N

30.7 ± 11.1

27.5 ± 9.7

35.1 ± 8.5*

31.3 ± 5.5*

-P

56.5 ± 10.7*

58.4 ± 11.7*

105.3 ± 15.3*

97.4 ± 10.1*

-N-P

52.1 ± 13.5*

56.6 ± 12.5*

98.5 ± 19.4*

111.5 ± 12.3*

  1. Cells were cultured as described in Table 2. Data are average ± 1 SD from five independent experiments. Asterisks indicate significantly higher levels (P < 0.01, two-tailed t-test) than that obtained from NORMAL nutrient condition with the same acetate supply.
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