Figure 4 | Scientific Reports

Figure 4

From: Use of Zebrafish Larvae as a Multi-Endpoint Platform to Characterize the Toxicity Profile of Silica Nanoparticles

Figure 4

Neurotoxicity of silica NPs as measured by the photomotor response (PMR) assay.

PMR was conducted on 30hpf embryos exposed between 4hpf–30hpf to silica NPs using concentrations ranging from 50 mg/l to 200 mg/l. The locomotor behaviors of embryonic zebrafish within the chorion was divided into the pre-stimulus (PRE), latency (L), excitation (E1, E2, E3) and refractory (R1, R2, R3) phases. 100 μM isoprenaline was used as a positive control. Each well of a 96-well plate contained five fish and three to six wells were used per condition. Raw data of total movement per well were used (expressed in motion units) and the data obtained from the different wells per condition averaged. Data represent the means and standard deviations for three independent biological replicates. (*p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA, Bonferroni correction).

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