Figure 2

Growth curves for RA ATCC11845, RA ATCC11845ΔtonB1 and the corresponding complementation strains in TSB medium (A) and TSB medium with 10 μM bovine hemoglobin (B). Cells were grown in 20 ml TSB medium or TSB with 10 μM bovine hemoglobin at 37 °C starting at OD600 = 0.1. OD600 values were then measured every 2 h for 14 h. The data were analyzed using two-way ANOVA. The error bars represent the standard deviation of three independent experiments.