Figure 5 | Scientific Reports

Figure 5

From: Investigation of TbfA in Riemerella anatipestifer using plasmid-based methods for gene over-expression and knockdown

Figure 5

Hemin transport activity assays for RA ATCC11845 ∆tonB1∆tonB2pLMF03, RA ATCC11845 ∆tonB1∆tonB2pLMF03::tonB1, RA ATCC11845 ∆tonB1∆tonB2pLMF03::tonB2 and RA ATCC11845 ∆tonB1∆tonB2pLMF03::tbfA.

The above strains were tested for hemin utilization efficiency on LB plates as described in the Materials and Methods section. After 48 h of growth, the diameter of the zone of turbidity in each well was measured in quadruplicate for each plate, and the mean diameter was calculated. The results are expressed as the mean ± SD of the diameters (in mm) obtained for the three plates. NG: No growth in the well. The data were analyzed using Student’s t-test.

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