Figure 4
Inactivation of JNK relieved insulin resistance induced by 11β-HSD1 overexpression.
(a) 3T3-L1 cells with or without 11β-HSD1 overexpression were transfected with DN-JNK or control plasmid and expression of p-JNK/JNK was assayed. The gels were run under the same experimental conditions. Shown are cropped gels/blots (The gels/blots with indicated cropping lines are shown in Supplementary Fig. S1). (b) Differentiated 3T3-L1 cells were transfected with DN-JNK or control plasmid and then incubated with TNF-α (1 μg/mL) for 6 h. Following TNF-α treatment, glucose uptake rate was detected. (c) Cells expressing DN-JNK were treated with TNF-α (1 μg/mL) for 15 min to assess the level of p-Akt/Akt. (d) 11β-HSD1 overexpressing cells were transfected with DN-JNK and incubated with insulin (100 nM) for 15 min. Following insulin treatment, glucose uptake rate was calculated. (e) Level of p-Akt/Akt in cells treated as in D. The gels from Fig. 4C and Fig. 4E were run under the same experimental conditions, respectively. Shown are cropped gels/blots (The gels/blots with indicated cropping lines are shown in Supplementary Fig. S1). *P < 0.05, **P < 0.01, ***P < 0.001; all data from 3 independent experiments.
