Figure 8
HuR directly binds to SPRY4-IT1 and β-catenin in HTR-8/SVneo cells.
(A) RIP with rabbit monoclonal anti-HuR and preimmune IgG from HTR-8/SVneo cell extracts. RNA levels in immunoprecipitates were determined by qPCR. Expression levels of SPRY4-IT1 RNA are presented as fold-enrichment in HuR relative to IgG immunoprecipitates. (B) U1 binding with SNRNP70 is a positive control. (C) RIP with rabbit monoclonal anti-HuR and preimmune IgG from HTR-8/SVneo cell extracts. RNA levels in immunoprecipitates were determined by qPCR. Expression levels of β-catenin RNA are presented as fold-enrichment in HuR relative to IgG immunoprecipitates. (D) U1 binding with SNRNP70 is a positive control.
