Figure 3
From: Classification of Tandem Mass Spectra for Identification of N- and O-linked Glycopeptides
Prediction of the glycosylation type.
The MS/MS spectrum corresponding to the following GPSM is shown: AHEVSEISVRTVYPPEEETGER – N3H3F0S0. The glycan composition of this GPSM can represent an N- or O-linked structure. The intensity of the oxonium ions, particularly the high ratio of 186 to 204 implies the glycosylation type to be O-linked. This prediction is in agreement with the peptide sequence, which lacks an N-glycosylation site and can only be O-glycosylated. The blue spectral lines correspond to unmatched peaks.
