Table 1 Glycopeptides identified using two different search approaches. Conventional: all oxonium ions spectra were searched for glycopeptides.

From: Classification of Tandem Mass Spectra for Identification of N- and O-linked Glycopeptides

  

Assigned MS/MS spectra

Unique glycopeptides

Peptide backbones

Glycan compositions

Accuracy of classification

N-glycopeptides

Conventional

1065

403

98

63

99.7%

Pre-classification

1481

529

123

80

100%

O-glycopeptides

Conventional

200

32

4

31

96%

Pre-classification

193

32

4

31

100%

  1. Pre-classification: oxonium ions spectra were separated by classification model and searched for respective N- and O-glycopeptides. The results show that pre-classification of the oxonium ion containing spectra improves the identification of N-glycopeptides, however it does not impact the identification of O-glycopeptides.
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