Figure 4
Pathogenicity and growth of Erwinia amylovora wild type and mutant strains.
(A,B) Symptoms caused by wild type, the csrB and csrA mutants and their complementation strains on immature pear fruits. Immature pears were surface sterilized, pricked with a sterile needle and inoculated with 2 μL of bacterial suspensions. Symptoms were recorded and photos were taken at 4 and 7 days post-inoculation (dpi). (C) Growth of Erwinia amylovora wild type, mutants and complementation strains. Immature pears were surface sterilized, pricked with a sterile needle and inoculated with 2 μL of bacterial suspensions. Tissue surrounding the inoculation site was excised with a cork borer no.4 and homogenized in 1 mL of 0.5x PBS. Bacterial growth within the pear tissue was monitored after 1, 2 and 3 days post inoculation by dilution plating on LB with appropriate antibiotics.
