Figure 3
From: mTORC1 is Required for Brown Adipose Tissue Recruitment and Metabolic Adaptation to Cold
Loss of mTORC1 affects substrate partitioning in BAT.
Control and Ad-RaptorKO mice were exposed to cold (10 °C) during 2 weeks and fasted for 6 hours before dynamic PET scan procedures. (A) Representative PET images of extraction coefficient uptake of the glucose analog 18FDG between 25–30 min post-injection (last frame). White arrows point to the interscapular BAT that is highlighted in the upper right insert. (B) (Left) Glucose extraction coefficient was determined using the Patlak graphical analysis following a 30-minutes dynamic scan. (Right) Total dynamic glucose uptake corrected for tissue weight was calculated from the extraction coefficient, BAT weight and plasma glucose levels (n = 4–5; mean +/− SEM; t-test; *P < 0.05; ***P < 0.001). (C) Gene expression (left) (n = 4; mean +/− SEM; Two-way ANOVA; ***P < 0.01 vs. Warm; ###P < 0.001 vs. Control) and circulating levels (right) of FGF21 (n = 8; mean +/− SEM; t-test; **P < 0.01). (D) Representative PET images of the uptake of the fatty acid analog 18FTHA between 25–30 min post-injection (last frame). White arrows point to the interscapular BAT that is highlighted in the upper right insert. (E) (Left) NEFA extraction coefficient was determined using the Patlak graphical analysis following a 30-minutes dynamic scan. (Right) Total dynamic NEFA uptake corrected for tissue weight was calculated from the extraction coefficient, BAT weight and plasma NEFA levels (n = 4; mean +/− SEM; t-test; *P < 0.05; ***P < 0.001).
