Figure 1 | Scientific Reports

Figure 1

From: The disruption of GDP-fucose de novo biosynthesis suggests the presence of a novel fucose-containing glycoconjugate in Plasmodium asexual blood stages

Figure 1

PfGMD transfection construct and integration events.

(A) Schematic representation of the transfection plasmid (pHH1-PfGMD) used to target and disrupt GMD gene in P. falciparum 3D7 parasites (3D7) and the expected recombination event (3D7∆GMD). The small white box in front of F1 fragment represents the absence of the first two ‘AT’ nucleotides in the start codon. The black boxes represent upstream and downstream DNA sequence flanking the PfGMD gene locus. The position of HindIII (H) and EcoRI (E) restriction sites, the position of GMD probe (thick black line) and the predicted length of the restriction fragments (thin black lines) are shown. (B) Southern Blot analysis of EcoRI and HindIII digested genomic DNA from 3D7 and 3D7ΔGMD parasites. Hybridisation of GMD probe to digested DNA from 3D7ΔGMD revealed restriction fragment sizes consistent with the disruption of PfGMD by the single recombination of the plasmid. *Matches with the expected size of the episomic plasmid copy. The panel shows a cropped blot and full-length blot is shown in Supplementary data (Supplementary Fig. S3).

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