Figure 2 | Scientific Reports

Figure 2

From: The disruption of GDP-fucose de novo biosynthesis suggests the presence of a novel fucose-containing glycoconjugate in Plasmodium asexual blood stages

Figure 2

P. falciparum 3D7ΔGMD grow at comparable rates to 3D7 control cell lines.

(A) Synchronous ring-stage 3D7ΔGMD and 3D7 growth was monitored over two complete life cycles (96h) by flow cytometry. (B) Intraerythrocytic development (x1000 magnification) of wild-type (3D7) and PfGMD (ΔGMD) null mutants of P. falciparum 3D7 strain. Time indicates hours post-invasion of tightly (5 hours window) synchronised in vitro cultures by means of a combination of Percoll and sorbitol treatments. (C) Inhibition of 3D7 (dark grey bar) and 3D7ΔGMD (light grey bar) growth by a 3-h heat-shock at 41.5 °C. Values are the average of three independent replicas, with standard deviation, and represent percentage of growth relative to identical cultures not subjected to heat-shock. Heat-shock was performed when parasites were at the trophozoite stage and parasitemia was measured by FACS at the next generation. Statistical analysis, performed by applying one-way analysis of variance Tukey’s post-test, showed no significant differences.

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