Figure 1

Physiological parameters of D. tertiolecta under nitrogen depletion.
D. tertiolecta was cultured in 50-mL batch cultures supplemented with 5% CO2. Cell growth and media nitrogen concentration (a) was measured every day. Total organic carbon (b,c) and dry cell weight (d), chlorophyll (e) and photosynthetic yield (f) were measured at different time points (Day 0, 3, 5, 8, 12, 17) to illustrate the different phases of growth. Error bars represent standard deviations from three independent biological replicates. Asterisks indicate statistically significant differences between N-replete and N-deplete samples after two-tailed t-tests (*p value ≤ 0.05; **p value ≤ 0.01).