Figure 6
From: Digital Detection of Exosomes by Interferometric Imaging
Dilution curve of exosomes purified from HEK cell line and detected with SP-IRIS.
A good correlation can be observed for both capture antibodies CD63 (yellow line, R2 0.97) and CD81 (blue line, R2 0.93); no particles were detected on the negative control R IgG (red line). The bright area around the regression line corresponds to the 95% confidence interval. Based on line equations, limits of detection (LOD) were calculated, resulting in 5.07E + 09 particles/mL for CD63 antibody and 3.94E + 09 particles/mL for CD81 antibody.
