Figure 7
From: Digital Detection of Exosomes by Interferometric Imaging
(A) Western blot analysis of exosomes isolated from 1.8 ml of hCSF and neat hCSF: the exosomal markers TSG101 and flotillin marked the exosomes fraction and not neat CSF, cystatin C marked both fractions and calnexin was not detected. (B) Sucrose gradient fractions of exosomal preparations from hCSF were immunoblotted with the TSG101 monoclonal antibody. TSG101 marked the exosomes-positive fractions (corresponding to 1.13 and 1.14 g/mL sucrose). HEK cells lysate was used as positive control. Western blot gels have been cropped to show only the relevant protein bands. Complete figures can be found as Supplementary, Figure SI 7.
