Figure 3
The effect of sodium iodate on RPE cell migration.
(A) ARPE-19 cell migration after 5-day sodium iodate treatment was evaluated by scratch wound assay. Images were taken at time 0, 6, 12 and 24 hours. The wound area was measured by Image J software. The percentage migration was calculated by the average area reduction at 6, 12 or 24-hour as compared to time 0. The data represented was the mean of triplicated experiments ± standard deviation. Scale bar: 200 μm. ‘***’p < 0.001. (B) Transwell assay was used to determine the migration of ARPE-19 cells. Bottom membrane of the transwell was stained with 0.2% crystal violet after 5-day treatment of sodium iodate. The numbers of cells migrated to the bottom of the membrane among different treatment groups were counted. Scale bars: 100 μm. ‘***’p < 0.001. (C) The expression of epithelial-to-mesenchymal transition (EMT) marker protein (α-SMA, SNAIL and VIMENTIN) in ARPE-19 cell after 5-day sodium iodate treatment was evaluated by immunoblotting. Compared to the control group, the expressions of α-SMA, SNAIL and VIMENTIN were significantly reduced in the 5 and 10 mM treatment groups. β-actin was used as housekeeping protein for normalization. ‘*’p < 0.05; ‘***’p < 0.001. The samples in the gel are run under the same experimental conditions.
