Figure 6
The effect of sodium iodate on ER stress marker expression.
The expression of endoplasmic reticulum (ER) stress response gene and protein (GRP78, ATF6, PERK and HTRA1) in ARPE-19 cell after 5-day sodium iodate treatment was evaluated by Sybr green PCR and immunoblotting, respectively. (A) Sybr green PCR analysis showed that 5 and 10 mM sodium iodate significantly increased the expression of HTRA1 gene. GAPDH was used as housekeeping gene for normalization. (B) Immunoblotting analysis showed that 2 and 5 mM sodium iodate significantly increased the expression of PERK protein, whereas HTRA1 expression was significantly reduced in 5 and 10 mM treatment groups when compared to the control group. β-actin was used as housekeeping protein for normalization. ‘*’p < 0.05; ‘**’p < 0.01. The samples in the gel are run under the same experimental conditions.
