Figure 7
The effect of sodium iodate on autophagy pathway marker expression.
The expression of autophagy pathway gene (ATG5, BECN1 and MAP1LC3B) as well as LC3B protein in ARPE-19 cells after 5-day sodium iodate treatment was analyzed by Sybr green PCR and immunoblotting, respectively. (A) The expressions of ATG5, BECN1 and MAP1LC3B genes were not significantly altered among different sodium iodate treatment groups Housekeeping gene (GAPDH) was used for normalization. (B) The activated form of LC3B protein, LC3B-II, was elevated in 5 and 10 mM of sodium iodate treatment, indicating that sodium iodate activated the autophagy machinery. β-actin was used as housekeeping protein for normalization. The relative expression levels were compared to that of the control group. ‘*’p < 0.05; ‘**’p < 0.01. The samples in the gel are run under the same experimental conditions.
