Figure 3

Performance of the Lent-On-Plus LVs on sorted hMSCs.

(a) Plots showing eGFP expression before (top plots) and after (bottom plots) purification of the eGFP+ populations of CEETnl2 and CEETnl2Is2-transduced hMSCs using fluorescence-activated cell sorting (FACS) Aria II Flow Cytometer. A MOI = 0.4 was used to keep the percentage of transduced hMSCs below 15%. (b) After sorting, the hMSCs were kept in the absence of Dox for 8 days and then induced (+Dox) or not (−Dox) for additional 5 days and analyzed for eGFP expression. The Percentages (%) of eGFP+ cells in the different population are shown inside each plot. The MFI of the whole living cells (WLC) (used to measure fold induction) are indicated at the bottom of each plot. Fold induction and leaking of the CEETnl2- and CEETnl2Is2-transduced sorted populations are indicated in the table (bottom).