Figure 5

Performance of the Lent-On-Plus LVs on sorted hESCs.

(a) Plots showing eGFP expression before (Pre-sorting) and after (Post-sorting) purification of the eGFP+ populations of CEETnl2 and CEETnl2Is2-transduced hESCs using fluorescence-activated cell sorting (FACS) Aria II Flow Cytometer. A MOI = 30 was used to achieve over 35% eGFP+ cells and good expression levels. (b) Sorted hESCs were kept in the absence of Dox for 8 days and then induced (+Dox) or not (−Dox) for additional 5 days and analyzed for eGFP expression. The Percentages (%) of eGFP+ cells are shown inside each plot. The MFI of the whole living cells (WLC) (used to measure fold induction) are indicated at the bottom of each plot. Fold induction and leaking of the CEETnl2- and CEETnl2Is2-transduced sorted populations are indicated in the table (bottom).