Figure 3
Effect of exogenous cAMP on LRP reactivated L. donovani.
(a) Effect of increasing concentrations of exogenous cAMP on percent motility of reactivated cells with time. (b) Effect of increasing concentrations of exogenous cAMP on the waveform proportions of LRP reactivated L. donovani. Cells were reactivated in LRP in presence of increasing concentrations of cAMP and the proportions of motile cells with flagellar and ciliary waveforms were quantified over 0, 5 and 10 min. (c) Reversibility of the effect of cAMP on waveform of LRP reactivated parasites. LRP → LRP denotes LRP reactivated parasites were diluted 10 times in LRP. 0.5 μM cAMP → 0.5 μM cAMP denotes LRP reactivated parasites in presence of 0.5 μM cAMP were diluted 10 times in LRP containing 0.5 μM cAMP. 0.5 μM cAMP → LRP denotes LRP reactivated parasites in presence of 0.5 μM cAMP were diluted 10 times in LRP (without cAMP). (d) Percent motility of cells reactivated in LRP (with or without 0.5 μM cAMP) and then diluted 10 times in ‘c’. (e) Chemical specificity of LRP reactivated cells towards cAMP. Distribution of reactivated cells with flagellar and ciliary waveforms plotted at 0 min time point in presence of cAMP, cGMP, pCPT-cAMP, AMP and adenosine. LRP-N denotes reactivation in presence of 10 mM NH4OH. Adenosine was dissolved in NH4OH. Red denotes proportion of motile cells with ciliary waveform. Blue denotes proportion of motile cells with flagellar waveform. All experiments were performed three times. Values are mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.
