Figure 5
Effect of endogenous cAMP levels on LRP reactivated L. donovani.
(a) Effect of dipyridamole (DPD) on proportions of reactivated cells with flagellar and ciliary beats. 0.1% DMSO control was maintained as DPD was dissolved in DMSO. Proportions of cells with ciliary and flagellar waveforms were quantified for each sample. (b) Effect of pre-treatment of live parasites with DPD. Parasites were pretreated with 25 μM DPD for 1 hour and subsequently reactivated in LRP without any exogenous cAMP. Proportions of cells with ciliary and flagellar waveforms were quantified for each sample. 1% DMSO control was maintained. (c) Intracellular cAMP concentration of live L. donovani parasites treated with or without 25 μM DPD for 1 hour. (d) Effect of endogenous cAMP levels on motility using phosphodiesterase enzyme (PDE) in LRP with time. A glycerol control was maintained as the enzyme was dissolved in 50% glycerol. (e) Effect of PDE on proportions of reactivated cells with flagellar and ciliary beats. Red denotes proportion of motile cells with ciliary waveform. Blue denotes proportion of motile cells with flagellar waveform. All experiments were performed three times. Values are mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.
