Figure 6
Effect of PKA inhibition on the distribution of reactivated L. donovani with flagellar and ciliary waveforms.
(a) Effect of H89 on proportions of reactivated cells with flagellar and ciliary beats. Cells were reactivated in LRP in presence of increasing concentrations of H89 and the proportions of motile cells with flagellar and ciliary waveforms were quantified over 0, 5 and 10 mins. (b) Effect of PKI on proportions of reactivated cells with flagellar and ciliary beats. Cells were reactivated in LRP in presence of increasing concentrations of PKI and the proportions of motile cells with flagellar and ciliary waveforms were quantified over 0, 5 and 10 mins. In ‘a’ and ‘b’, 0.1 μM cAMP was added to reactivated cells at 5 min time point shown with black arrows. All experiments were performed three times. Values are mean ± SEM.
