Figure 6
Imaging of mouse colonic dysplasia.
(A) White light image of CPC;Apc mouse colon collected in vivo with wide-field endoscope shows location of spontaneous adenoma (arrow). (B) Maximum uptake of the NIR-labeled EGFR peptide QRH*-Cy5.5 is seen from the adenoma ~90 minutes after systemic administration. From the in vivo confocal images, (C) increased fluorescence intensity is seen from cell surface of dysplastic colonocytes (arrow) while (D) minimal staining is seen in normal crypts. Real time video (visualization) of side-viewing endomicroscope moving from adenoma to normal is shown. (E) A significantly greater mean fluorescence intensity was measured from dysplasia compared with that of adjacent normal, 107 ± 19 versus 62 ± 8 AU, from a group of n = 6 mice, P = 3.5 × 10−4 by paired two-way t-test.
