Table 2 Strains and plasmids used in this study.

CMPG5300

Wild type, human vaginal isolate

Malik et al.²⁴

CMPG11201

Δcmpg5300.05_29::Cm^r

This study

CMPG11202

CMPG11201 complemented with cmpg5300.05_29

This study

WCFS1

Sequenced wild-type strain; single colony isolate of NCIMB 8826 from human saliva

48

TOP10F

F’ (lacIq, Tnr) mcrA_(mrr hsdRMS-mcrBC) 80LacZ_lacX74 deoR recA1 araD139_(araleu)

Invitrogen

BL21/DE3

E. coli B F– dcm ompT hsdS(r_B⁻ m_B⁻) gal λ(DE3)

Invitrogen

UTI89

Wild type, clinical isolate

49

P. aeruginosa

PA14

Wild type, human isolate

50

S. cerevisiae

BY 4741

MATa; his3Δ 1; leu2Δ 0; met15Δ 0; ura3Δ 0

51

C. albicans

SC5314 (ATCC MYA-2876)

Wild type, human clinical isolate

52

SH1000

rsbU positive derivative of S. aureus 8325-4

53

Rosenbach (ATCC 33591)

Wild type, clinical isolate

ATCC

ATCC 14028

Wild type, isolated from chicken tissue

ATCC⁵⁴

ATCC 14028 carrying pFPV25.1

Mutant constitutively expressing GFP

46

Host

Relevant description

Reference/source

E. coli

pNZ5318 derivative for multiple gene replacements in gram-positive bacteria Cm^r Ery^r

Lambert et al.⁴²

E. coli

pLAB1301 derivative driven by dlt promoter, Ap^r, Ery^r

Unpublished results

E. coli

pNZ5319 derivative containing homologous regions up- and downstream of cmpg5300.05_29, Cm^r Ery^r

This study

E. coli

pCMPG10208 derivative containing cmpg5300.05_29 of CMPG5300, Ap^r, Ery^r

This study

E. coli

Kan^r, T7 lac, N and C-terminal His Tag

Novagen

E. coli

pET28a+ derivative containing the lectin domain of cmpg5300.05_29 of CMPG5300, Kan^r

This study

E. coli

pET28a+ derivative containing the lectin domain of msa gene of L. plantarum WCFS1, Kan^r

This study