Figure 1 | Scientific Reports

Figure 1

From: Smart-aggregation imaging for single molecule localisation with SPAD cameras

Figure 1

(a) Schematic representation of the output of a binary SPAD imager. Each pixel registers a 0 (no photon count, white) or 1 (photon count, grey) in time. (b) Representation of the operational frame capture of an emCCD and bit-plane imager for an example, single blinking molecule. The top graph represents the molecule intensity trace in time, the middle graph the collected intensity from a fixed frame rate emCCD. The grey regions represent data readout sectors. The bottom graph shows the difference between fixed frame capture and smart-aggregated frame capture for a SPAD CMOS camera. (c) Simulated point spread functions from the molecule emitter in each time frame as imaged in the emCCD.

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