Figure 3
From: Smart-aggregation imaging for single molecule localisation with SPAD cameras

Single molecule localisation uncertainty for varying molecule blink durations as determined for (a) the SPAD SPCImager with and without smart-aggregation and (b) the emCCD with and without merged frames. Shown are both experimental data from GATTA-Paint 80 G nanorulers and simulated data. The solid lines are visual guides for the eye. (c) and (d) Shows histograms of estimated experimental blink duration of single molecules, as obtained from (c) SPCImager and (d) emCCD with 50 msec frame rate.