Table 2 ThunderSTORM molecule detection settings for simultaneous emCCD/SPCImager imaging.

From: Smart-aggregation imaging for single molecule localisation with SPAD cameras

Process

emCCD

SPCImager

Image filtering (default: Wavelet filter)

Wavelet filter

Gaussian filter (sigma = 2 pixels)

Peak intensity threshold (default: std(Wave.F1))

4*std (Wave.F1)

mean (Med.F) + std (Wave.F1)

  1. Wave.F1 is the first wavelet level of the input image, and Med.F is the median filtered input image.
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