Figure 3
Transmammary transfer of BMP9 and BMP10 blocking Abs induces AVMs in neonatal retinas.
(A,B) Representative images of blue latex-perfused retinal vasculature of P6 neonates fed for 3 days by dams injected on P3 with control IgG2a/b Abs (A) or BMP9/10 blocking Abs (B). Arrows in B indicate AVMs. Scale bars, 500 μm. (C) Scheme depicting the method employed for the quantification of the number of latex dye-positive vessels. (D) Histogram showing the total number of vascular crosses. (E) Histogram showing the number of vascular crosses per concentric circle on circles 1 to 3. Data represent mean ± s.e.m. (n = 5–7 pups per group from 2 dams); ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05; ANOVA and Kruskal-Wallis test (D,E).
