Figure 1 | Scientific Reports

Figure 1

From: Importance of the C-terminal histidine residues of Helicobacter pylori GroES for Toll-like receptor 4 binding and interleukin-8 cytokine production

Figure 1

Purification and functional characterization of WT and histidine mutant proteins.

(A) Schematic representation of the domain structures and domain B sequences (residues 91-118) of WT and histidine mutants. Mutation sites of histidine to alanine are underlined and marked in red. (B) FPLC profiles of WT and histidine mutant proteins on a size exclusion column, HiLoad 16/600 Superdex 200 pg. A280 was monitored to determine protein elution and purity. (C) Purified His-tagged fusion WT and histidine mutants were separated via 15% SDS-PAGE and stained with Coomassie Blue. A 1 μg protein sample was loaded from each elution fraction. (D) KATO-III cells were incubated for 16 h with 0.05 μM WT and histidine mutants, and IL-8 levels in the culture supernatant measured using the Quantikine ELISA assay kit. Data are presented as means ± SD of three experiments. *p < 0.05, compared to WT.

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