Figure 3 | Scientific Reports

Figure 3

From: Importance of the C-terminal histidine residues of Helicobacter pylori GroES for Toll-like receptor 4 binding and interleukin-8 cytokine production

Figure 3

Surface plasmon resonance (SPR) assay of specific binding affinities of WT and histidine mutants to immobilized TLR4.

After immobilization of TLR4 on the CM5 sensor chip surface, WT and histidine mutant samples were injected over immobilized TLR4 at a flow rate of 30 μl/min at 25 °C. PBS with 0.05% P20 running buffer was injected, and the response in resonance units recorded for 240 s as a function of time. Protein samples were replaced in running buffer for 480 s for disassociation of bound HpGroES. SPR sensograms were obtained after injection of serial dilutions of WT (A) and histidine mutants (B–I) (concentration: 0–1000 nM, top right) in running buffer. Data are representative of three independent experiments. The Kd and Bmax values of protein samples are shown in Table 2.

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