Figure 6

SAXS data analysis and ab initio models of WT and variant proteins.
(A) SAXS intensity profiles showing the log of X-ray scattering intensity (logI) as a function of the scattering vector q for experimental scattering data. The Guinier curve-fitted lines for Rg values to the experimental data are superimposed (Inset). Both curve profiles were corrected and offset for clarity of presentation, and showed good quality of protein samples. (B) Overlay of normalized p(r) distribution plot calculated using Primus for WT and variants. (C) The ab initio models of averaged molecular envelopes from DAMMIN (left) and rigid body models from CORAL (right) are shown in the same colors as those in panel A. The models showed good fit with the experimental SAXS curve in (A) (χ2 = 1.4–2.1).