Figure 2
CPD and Brdu expression in cultured keratinocytes after indicated UVB irradiation.
(A) The CPD lesions of normal keratinocytes at 4 and 6 hr after high irradiance (HI) and low irradiance (LI) UVB (23 mJ/cm2) treatment as determined by ELISA specific for CPD. (B) Double labeling of HaCaT keratinocytes 3 hr after HI and LI UVB (23 mJ/cm2) treatment with anti-CPD antibody (green) and anti-BrdU (red). (C) Double labeling of ERK inhibitor (U0126; 5 μM) pretreated HaCaT keratinocytes 3 hr after HI and LI UVB (23 mJ/cm2) treatment with anti-CPD antibody (green) and anti-BrdU (red). The numbers show percentage (mean ± SD) of BrdU + CPD keratinocytes.
