Figure 3
From: miR-96 promotes cell proliferation, migration and invasion by targeting PTPN9 in breast cancer
The effect of miR-96 on cell cycle progression in breast cancer cells.
(A–D) Cell cycle profiles were analyzed using flow cytometry after the transfection of MCF-7 cells with equal doses of miR-NC, miR-96, anti-miR-NC or anti-miR-96. The untransfected cells (Mock) serve as the negative control. The panel shows histograms of cell numbers (y axis) against DNA content (x axis) determined by measuring fluorescence intensity. Numbers denote the percentages of cells in the G1/G0, S and G2/M phases. (A and B) Representative images; (C and D) quantitative analysis. (E and F) Quantitative RT-PCR analysis of the relative expression levels of Cyclin D1, CDK6, CDK4 and p21 mRNA in MCF-7 cells transfected with equal doses of miR-NC, miR-96, anti-miR-NC or anti-miR-96. The untransfected cells (Mock) serve as the negative control. (G) Western blot analysis of Cyclin D1, CDK6, CDK4 and p21 protein levels in MCF-7 cells transfected with equal doses of miR-NC, miR-96, anti-miR-NC or anti-miR-96. The untransfected cells (Mock) serve as the negative control. *P < 0.05; **P < 0.01; ***P < 0.001.
