Figure 6
From: miR-96 promotes cell proliferation, migration and invasion by targeting PTPN9 in breast cancer
The effect of PTPN9 on the proliferation, migration and invasion of breast cancer cells.
(A–C) The EdU proliferation assay was performed 48 h after the transfection of MCF-7 cells with equal doses of siRNA-NC or PTPN9 siRNA, or with equal doses of plasmid-NC or PTPN9 overexpression plasmid. The untransfected cells (Mock) serve as the negative control. The cells with red fluorescence are in the S phase of mitosis, and the cells with blue fluorescence represent all of the cells. (A and B) Representative images, Scale bar = 300 μm; (C) quantitative analysis of EdU-positive MCF-7 cells. (D and E) Cell migration ability was analyzed using wound healing assays after the transfection of MCF-7 cells with equal doses of siRNA-NC or PTPN9 siRNA, or with equal doses of plasmid-NC or PTPN9 overexpression plasmid. (D) Representative images, Scale bar = 100 μm; (E) quantitative analysis s of wound closure. (F–I) Cell invasion ability was analyzed using transwell assays after the transfection of MCF-7 cells with equal doses of siRNA-NC or PTPN9 siRNA, or with equal doses of plasmid-NC or PTPN9 overexpression plasmid. The untransfected cells (Mock) serve as the negative control. (F and H) representative images, Scale bar = 250 μm; (G and I) quantitative analysis. **P < 0.01; ***P < 0.001.
