Figure 5 | Scientific Reports

Figure 5

From: ECM proteins in a microporous scaffold influence hepatocyte morphology, function, and gene expression

Figure 5

Immunofluorescence staining of CYP3A4 protein in Huh-7.5 cells on (a) the bare PEG-DA scaffold, (b) the collagen-functionalized scaffold, and (c) the fibronectin-functionalized scaffold, on days 1, 7, and 14. CYP3A4 was stained with Alexa Fluor 488 (green), F-actin was stained with Alexa Fluor 555 phalloidin (red), and cell nuclei were counterstained with DAPI (blue). High quality figures of the day 14 merged images are shown in Supplementary Fig. S1.

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