Table 2 Primers used in this research.
Primer | Primer sequence |
|---|---|
For quantitative real-time PCR | |
QNlHsp70-F | 5′-CGATGAGGGCTCTCTATTTG-3′ |
QNlHsp70-R | 5′-CTCGTGGGTTGGACTTGAT-3′ |
QNlAcc-F | 5′-TTACTGATGGCTTGGCTAC-3′ |
QNlAcc-R | 5′-CGACATTACGACCCTGAC-3′ |
For Hsp70 and ACC dsRNA synthesis | |
NlHsp70-F | 5′-CTGACCATCGAGGATGGCATCT-3 |
NlHsp70-R | 5′-ACCGCCTCGTCGGGATTGAT-3′ |
NlAcc-F | 5′-TTACTGATGGCTTGGCTAC-3′ |
NlAcc-R | 5′-CGACATTACGACCCTGAC-3′ |
For Gfp dsRNA synthesis | |
Gfp-F | 5′AAGGGCGAGGAGCTGTTCACCG-3′ |
Gfp-R | 5′-CAGCAGGACCATGTGATCGCGC-3′ |
