Table 1 primers and plasmids used for qseC mutation in this study.
Primer | Sequence | |
|---|---|---|
qseC up | Forward | 5′-CACAGTGCCATAACGGCAACG-3′ |
Reverse | 5′-GAAGCAGCTCCAGCCTACACTCATGCGTCACCCAGGGTGT-3′ | |
qseC down | Forward | 5′-CTAAGGAGGATATTCATATGCAGAGACTTTTGCCAAAAACGC-3′ |
Reverse | 5′-GAGGACGGCCTGACGGTGATGT-3′ | |
cm | Forward | 5′-ACACCCTGGGTGACGCATGAGTGTAGGCTGGAGCTGCTTC-3′ |
Reverse | 5′-GCGTTTTTGGCAAAAGTCTCTGCATATGAATATCCTCCTTAG-3′ | |
Plasmids | Description | |
pKD3 | pANTSγ derivative containing FRT-flanked chloramphenicol resistance | |
pKD46 | A red recombinase expression plasmid | |
pCP20 | An ampicillin and CmR plasmid that shows temperature-sensitive replication and thermal induction of FLP synthesis | |
