Figure 5

Heterogeneity of CDP division kinetic and cell fate.

CDP and their progeny were monitored by time-lapse microscopy and single cell tracking. Cell divisions and fluorescent signals were annotated in pedigrees. Pedigrees were segregated according to their cell fate. Examples of pedigrees with pDC and SiglecH⁺ CCR9^low fate (A), with only SiglecH⁺ CCR9^low fate (B,C) or with SiglecH⁺ CCR9^low and cDC fate (D) are shown. (E) The pie chart shows the percentages of pedigrees, in which generation of pDCs or cDCs occurred, in which SiglecH⁺ CCR9^low precursor cells were generated without occurrence of pDCs or cDCs, and, in which only cells with CDP or pre-DC phenotype were generated (undifferentiated).