Figure 5
Divergent expression profiles of the genes involved in terpenoid backbone biosynthesis and the ABA biosynthetic gene cluster in TBC-A and TBC-6 under identical fermentation conditions.
(A) Schematic representation of the transcript abundance of terpenoid backbone biosynthetic genes in TBC-A and TBC-6 samples. Red boxes indicate increased gene expression, blue indicates repressed gene expression and gray indicates no significant differences were observed in TBC-A samples compared to TBC-6 samples. The numbers in the boxes correspond to the EC (Enzyme Commission) numbers, and the expression levels of the genes encoding these enzymes were calculated as RPKM values in the samples TBC-6-41 h, TBC-6-72 h, TBC-6-120 h,TBC-A-41 h, TBC-A-72 h and TBC-A-120 h. The dotted arrows and boxes indicate enzymatic steps or compounds that were not detected. The expression patterns of the 4 genes located in the ABA biosynthetic gene cluster were evaluated for TBC-A (B) and TBC-6 (C) during the fermentation process. Relative expression levels of bcaba1 (D), bcaba2 (E), bcaba3 (F) and bcaba4 (G) transcripts in the 12 culture samples of TBC-A and TBC-6 are shown. EC 2.3.1.9: acetyl-CoA acetyltransferase; EC 2.3.3.10: hydroxymethylglutaryl-CoA synthase; EC 1.1.1.34: 3-hydroxy-3-methylglutaryl-CoA reductase; EC 2.7.1.36: mevalonate kinase; EC 2.7.4.2: phosphomevalonate kinase; EC 4.1.1.33: diphosphomevalonate decarboxylase; EC 5.3.3.2: isopentenyl-diphosphate delta-isomerase; EC 2.5.1.10: farnesyl pyrophosphate synthase; EC 2.5.1.29: geranylgeranyl diphosphate synthase; EC 2.5.1.21: squalene synthase.
