Figure 3
From: Characterization of two distinct early post-entry blocks to HIV-1 in common marmoset lymphocytes
The post-entry block(s) to HIV-1 in marmoset PBLs and B-LCLs are dominant.
(A) Heterokaryons or homokaryons of human (hu) and marmoset (mar) PBLs were produced by treatment with PEG. As a control, mixtures of human and marmoset cells were treated the same way but without adding PEG. The fused and non-fused cell mixtures were challenged with HIV-1 luciferase reporter viruses pseudotyped with VSV-G. Forty-eight hours later, the infectivity of the viruses was determined by measuring the relative luciferase activity in the cells. The infectivity in the human-human homokaryons is normalized to 100%, and the relative infectivity in the other homo-heterokaryons is shown. The results of four (+PEG) and two (no PEG) independent experiments and their means are shown. (B) Human and marmoset B-LCLs were fused with PEG to generate hetero- or homokaryons. As a control, mixtures of human and marmoset cells were treated the same way but without adding PEG. The fused and non-fused cell mixtures were challenged with HIV-1 luciferase reporter viruses pseudotyped with VSV-G. Forty-eight hours later, the infectivity of the viruses was determined by measuring the relative luciferase activity in the cells. The infectivity in the human-human homokaryons is normalized to 100%, and the relative infectivity in the other homo-heterokaryons is shown. The results of three independent experiments and their means are shown. Reported p-values (2-tailed) were obtained with an unpaired t-test.
