Figure 6
From: Characterization of two distinct early post-entry blocks to HIV-1 in common marmoset lymphocytes
HIV-1 is blocked in marmoset B-LCLs after reverse transcription.
Human (hu) or marmoset (mar) B-LCLs were challenged with single-cycle HIV-1 GFP reporter viruses pseudotyped with VSV-G (indicated as WT), or as a control with viruses lacking an envelope glycoprotein (ΔEnv). Viruses were pretreated with DNase I for 1 h. Two hours post-infection (p.i.), the cells were thoroughly washed 3 times with PBS. At different time points after infection, cells were collected and total DNA isolated. The amounts of early (A) and late (B) RT products and 2-LTR circles (C) were quantified by real-time qPCR using TaqMan® chemistry. ND, not detectable. The results of two independent experiments are shown.
