Figure 7
From: Characterization of two distinct early post-entry blocks to HIV-1 in common marmoset lymphocytes
Infectivity of HIV-1 capsid mutants in B-LCLs.
(A) Human (hu) or marmoset (mar) B-LCLs were challenged with single-cycle HIV-1 luciferase reporter viruses pseudotyped with VSV-G and containing the indicated changes in their capsids. Forty-eight hours later, the infectivity of the viruses was determined by measuring the relative luciferase activity (RLU) in the cells. The results of three or five independent experiments and their means are shown. The mean fold change in infectivity for each mutant relative to WT virus in the marmoset B-LCLs is shown above the chart. NI, non-infected. (B,C) Human (hu) or marmoset (mar) B-LCLs were challenged with the single-cycle HIV-1 N74D capsid mutant as in the experiments shown in Fig. 6A,B and C. At different time points after infection, cells were collected and total DNA isolated. The amounts of late RT products (B) and 2-LTR circles (C) were quantified by real-time qPCR using TaqMan® chemistry. The results of two independent experiments are shown. The inserts in panel B show the amount of viral cDNA six (left) or seven (right) days post-infection (integrated proviruses) for the WT and N74D viruses. ND, not detectable.
