Figure 8
From: Characterization of two distinct early post-entry blocks to HIV-1 in common marmoset lymphocytes
Characterization of the post-entry block to HIV-1 in B-LCLs.
Human (hu) or marmoset (mar) B-LCLs were challenged with single-cycle HIV-1 WT or N74D capsid mutant luciferase reporter viruses pseudotyped with VSV-G. (A) Cells were pre-treated with 500 U/ml of universal IFN-α for 24 hours before the infection. (B) Cells were pre-treated with 4 μM As2O3 for 3 hours before the infection. The cells were maintained in the presence of the treatment for 24 hours after the viral challenge. Forty-eight hours post-challenge, the infectivity of the viruses was determined by measuring the relative luciferase activity (RLU) in the cells. The results of five independent experiments are shown. Reported p-values (2-tailed) were obtained with a paired t-test (untreated versus treated cells). NT, no treatment.
