Figure 4 | Scientific Reports

Figure 4

From: The coordinated roles of miR-26a and miR-30c in regulating TGFβ1-induced epithelial-to-mesenchymal transition in diabetic nephropathy

Figure 4

Co-treatment with the miR-26a and miR-30c inhibitors enhances TGFβ1-induced EMT.

(A) Western blot analyses of FN, Col-I, E-cadherin and α-SMA in NRK-52E cells transfected with antimiR control, antimiR-26a, antimiR-30c or a combination of antimiR-26a and antimiR-30c (each at half the dose). The results showed significant increases in FN, Col-I, and α-SMA and a reduction in E-cadherin protein expression. (B) Relative protein abundance of FN, Col-I, α-SMA and E-cadherin. (C) qRT-PCR. The bars represent the mean ± SEM (n = 3). The data are presented as the mean ± SEM (n = 3). *p < 0.05 versus NC; #p < 0.05 versus TGFβ1; $p < 0.05 versus miR-26a inhibitor + TGFβ1; &p < 0.05 versus miR-30c inhibitor + TGFβ1.

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