Table 2 Primers used for quantitative and semi-quantitative RT-PCR.

From: Dopamine regulates renal osmoregulation during hyposaline stress via DRD1 in the spotted scat (Scatophagus argus)

Primer name

Primer sequences

Product size (bp)

SaDRD1-semi-F

5′-CCATCTCAAGCCCATTTCGC-3′

542

SaDRD1-semi-R

5′-GGCACCTTCAGGCAAGTTCG-3′

 

SaDRD1-qRT-F

5′-AGCCCATTTCGCTATGAACGCA-3′

192

SaDRD1-qRT-R

5′-TCAGGCTGGAATCGCAGTTGTC-3′

 

SaDRD5-qRT-F

5′-CATGGTGGTGTCGTGGTGAT-3′

142

SaDRD5-qRT-R

5′-CCCGGTCATTTACGCCTTCA-3′

 

NKA α1-qRT-F

5′-AGCTGAAAGACATGACCGCA-3′

183

NKA α1-qRT-R

5′-TGTCAGCCTTCTTCAGAGCG-3′

β-actin-F

5′-TAGTCTGTGAGGTCACGG-3′

151

β-actin-R

5′-CTGTGCTGTCCCTGTATG-3′

  1. B = C/G/T, H = A/T/C, K = G/T, M = A/C, R = A/G, S = C/G, V = A/C/G, W = A/T, Y = C/T.